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The rest of the binding sites non-specifically interacted with other slightly charged proteins, such as histidine-rich protein, leading to impurities later on

Effect of reduced glutathione (GSH) in canine sperm cryopreservation: in vitro and in vivo evaluation

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Secondary antibodies were added in 10 ml of blocking buffer and incubated with membrane with gentle agitation for 1 hour at room temperature

(Wilmington, MA, USA) were maintained and bred at the animal house of the Fondazione IRCCS Carlo Besta Neurological Institute, in compliance with institutional guidelines and international regulations (EEC Council Directive 86/609)