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Reactive oxygen species cause oxidation that exceeds the capacity of antioxidant defense system, leading to oxidative stress (26)

For the index PCR, a 25 L reaction containing amplicon PCR product, 0.5 L of Platinum SuperFi II DNA Polymerase, 1 SuperFi II Buffer (Thermo Fisher Scientific), and 0.5 M each of Nextera XT Index Kit v2 index primers (Illumina) was prepared

However, only one of the two major cellular ROS degrading regulators, SoxR (PP_2060 log 2 -FC 1.1), was found to be marginally up-regulated

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