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However, isotope-labeled IS are often expensive or unavailable for certain compounds and using a single isotope-labeled IS for multiple structurally diverse compounds is inappropriate due to differences in retention times, matrix effects, ion suppression, fragmentation patterns, and ionization efficiencies

The progressive identification of these changes has led to the development of the concept of leaky gut syndrome and gut dysbiosis, which underlie the relationship between intestinal barrier impairment, metabolic diseases and autoimmunity

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There are two types of GHS studied in the scientific literature: peptide GHS and non-peptide compounds