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After incubation at 4 C for 30 min, the reaction mixtures were added to 100 L of 50% (v/v) Ni-NTA superflow resin slurry that was pre-equilibrated with buffer containing 50 mM BisTris, pH 6.8, 100 mM NaCl, 25 mM imidazole, and 10% glycerol

While it might not be the quickest option, consulting with your trusted physician is a great way to make sure youre making an informed and safe decision for your body

PCR amplification of bacteria DNA was performed as previously described 46

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Vitamin B12 is combined with a protein called intrinsic factor in your stomach