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Final coupling: After completing the coupling of all amino acids, 2- (tert butoxycarbonylamino) isobutyric acid is added for final coupling, which further improves the structure of the peptide chain

1 H-NMR (300 MHz, CDCl 3 ) 4.04 (t, J=6.3 Hz, 4H), 3.54 (m, 2H), 2.95-2.92 (m, 2H), 2.61-2.22 (m, 6H), 1.85-1.75 (m, 1H), 1.76-1.12 (m, 66H), 0.87 (t, J=6.9 Hz, 12H), 0.55-0.42 (m, 1H)

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In one small proof-of-principle physiology study, untreated hypogonadal men had an intact IGF1 response to GH stimulation, and this response was unchanged by cross-over treatment with both testosterone and dihydrotestosterone administered as transdermal gel (Veldhuis et al., 2009)