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Interestingly, GSAAT(WT), GSAAT(C138S) and GSAAT(C396S) lines were still markedly redox responsive, and exhibited up to 20% higher enzyme activity when supplied with DTT relative to the UT control conditions, while the two gsa2 lines expressing GSAAT(C168S) and GSAAT(C190S) resulted only in 9% and 2% increased GSAAT activity upon addition of DTT to the extracts ( Supplementary Figure 3E )

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Low levels of intracellular calcium from low frequency stimulation activates protein phosphatases, which leads to decreased conductance of AMPA receptors and removal of AMPA receptors in the cell membrane

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SOD, superoxide dismutase Acknowledgements: This study was supported by grants from the Jagiellonian University Medical College in Cracow, Poland