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PCR conditions were as following: initial denaturation at 95 C for 5 min, followed by 37 cycles of denaturation (94 C for 40 s), annealing (57 C for 30 s) and extension (72 C for 40 s), with the final extension at 72 C for 10 min

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[DOI] [PMC free article] [PubMed] [Google Scholar] 128.Porcu M., Urbano M.R., Verri W.A., Jr, Barbosa D.S., Baracat M., Vargas H.O., Machado R.C.B.R., Pescim R.R., Nunes S.O.V

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Providing patients with an external air supply during the procedure