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After 60 min incubation the samples were washed 3x with 1 ml of binding buffer (20 mM HEPES-KOH (pH = 7.9), 150 mM KCl, 0.2 mM EDTA, 1 mM DTT 15 % glycerol, 0.2 % NP-40), mixed with 800 l of RNase-treated soluble nuclear lysate prepared from HCT116 cells (see protocol above) and rotated for 60 min at 4 C

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Src activation by SH3/SH2-binding proteins is well established: binding to these domains disrupts the intramolecular interactions that constrain the kinase activity 36,37,38,39,40,41,42

S., Lalli, E