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tissue sample: 30-50 g per well) were separated by electrophoresis on a 10% SDS-polyacrylamide gel and transferred to a PVDF membrane

doi: 10.1016/j.cellsig.2024.111239 59 OkazakiSUmeneKYamasakiJSuinaKOtsukiYYoshikawaMet al

doi: 10.1016/j.cellsig.2010.06.004

Some approaches being explored include nanoencapsulation using broccoli membrane vesicles, which has shown improved cellular uptake and metabolism of SFN in cancer cells