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Description

J.SpiersJ

[4] (TSSO)

The TMD is responsible for an anchoring of the envelope proteins in the membrane of the ER and their ER retention[32]

Pucino, V., Bombardieri, M., Pitzalis, C

You wont want to expose your protein of interest or the interacting proteins to 20% ethanol, so after adding your beads to a plastic column youll first rinse the beads out with deionized water (dIH 2 O)