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But for new users, seeing pink liquid in a syringe can feel different than seeing clear liquid, even though the technique is identical

8 mM of reducing agent DTT (Sigma) was then added to the samples and incubated in a thermomixer at 30C for 30 minutes, followed by the addition of 20 mM iodoacetamide (Sigma) for protein carboxylation with incubation at 30C in the dark for 30 minutes

After staining with crystal violet and subsequent destaining, absorbance was recorded at 517 nm using a spectrophotometer, following the protocol described by [19, 23]