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coli extract and the development of a fully purified transcription and translation platform (PURE: Protein synthesis Using Recombinant Elements) in the early 2000s 26 led to an explosion of research applications enabled by rapid protein synthesis in vitro

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doi: 10.1016/j.bjoms.2009.08.001

77 In summary, rRNA modifications are integral to regulating translation efficiency, optimizing protein synthesis, and affecting the growth of organisms as well as the onset of diseases

A unique gain factor is then calculated for each sample (represented by a scalar multiplier k) and compound ion abundances were adjusted to a normalization reference run 68