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6A-C, compared with the Control group, the DSS group exhibited significantly increased ROS levels, decreased GSH levels, and elevated MDA levels in colonic tissues, indicating that DSS induced oxidative stress and lipid peroxidation

Representative tissue specimens were embedded in paraffin, sectioned at 4 m, stained with hematoxylin and eosin (H&E), and evaluated by light microscopy using an Olympus 71 digital camera and an Olympus BX51 microscope (Japan) acquiring digital images saved as uncompressed 24-bit RGB TIFF files