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Kiyohara ACP, Torres DJ, Hagiwara A, Pak J, Rueli R, Shuttleworth CWR, Bellinger FP

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For reduction and alkylation, 20 l of each sample corresponding to 40 g of protein was mixed with 20 l of a solution of 40 mM CAA20 mM TCEP and incubated at 37 C for 30 min