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This leads to drastically reduced proliferative capacity and cytotoxicity in tumor-infiltrating CD8 + T cells and NK cells due to insufficient metabolic substrates, rendering them poorly responsive to immune checkpoint blockade

For each independent in vitro experiment, at least three biological replicates were used

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Ma and Xu, 2013)

Together, these strategies aim to build an integrated framework spanning mechanistic studies, targeted delivery systems, and clinical validation, ultimately advancing ferroptosis-based therapies toward precision treatment for ALS