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The wild-type is able to advert immune system by having all these properties and this will delay the activation of macrophages and the downstream signaling pathways ( BacA has been found to impair MAP growth in vivo ( BacA is not over-attenuated to get cleared before inducing immune responses, and it is not under-attenuated to persist in tissue and maintain a chronic infection

EFCK3 ( Figure 2d ), 19 DEGs overlapped, and 10 upregulated ones except for TRINITY_DN4455_c0_g4 had higher expression levels on day 2, whereas nine DEGs were reversed in trend in both groups

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In one aspect, this invention provides methods for improving the efficacy of genetic vaccines by providing materials that facilitate targeting of a genetic vaccine to a particular tissue or cell type of interest