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Quantitative PCR was performed with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, Waltham, MA, USA) on a Mastercycler nexus X2 (Eppendorf, Hamburg, Germany)

- Liquidliquid extraction: this method involves using solvents to selectively extract specific compounds from OMWW, such as polyphenols, which can then be further processed and purified

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Aslanbay Guler B, Saglam-Metiner P, Deniz I, Demirel Z, Yesil-Celiktas O, Imamoglu E (2023) Aligned with sustainable development goals: microwave extraction of astaxanthin from wet algae and selective cytotoxic effect of the extract on lung cancer cells