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Anim Biosci 37:106576

Furthermore, persisters frequently display upregulation of the ROS-detoxification machinery and stress-protective storage molecules (trehalose, glycogen), which together blunt the lethal effects of fungicide-induced oxidative damage and support recovery once the drug pressure subsides (Wuyts et al., 2018)

564 Likewise, primary astrocytes prepared from ALS post-mortem samples induced necroptosis of neurones in co-culture, 565 and transplantation of diseased astrocytes into the spinal cord triggered motor deficits and promoted motor neurones degeneration

Methods Samples used to determine the number of sub-visible particles were stored at stressed conditions, defined as: - Duration: 28 days - Temperature: 30C 2C - Stress condition: During storage, samples were inverted 360 to simulate patient use out of refrigerated storage

Future studies should consider incorporating complementary methods, such as p62/SQSTM1 or electron microscopy, to achieve a more accurate and comprehensive evaluation