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Acknowledgements We thank the technical laboratory Lvia Simes-Ambrosio, University of Sao Paulo, Ribeiro Preto, for help in the analysis of Hcy, vitamin B 12 and folate

Ng et al., 2021)

Through literature research we learned that 6x his-tag are more commonly used and easier to elute off a column compared to a 10x his-tag (Carlsson et al., 2016)