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SDS-PAGE Protein samples (2 g) were analyzed on 12% Bis-Tris Bolt SDS-PAGE gels (ThermoFisher) with Bolt MES SDS running buffer (ThermoFisher)

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(I, J) Mitochondrial membrane potential levels in parental and EGR1-upregulated MDA-MB-231 and MDA-MB-468 cells treated with or without ferroptosis inducer erastin were detected by TMRE assay