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If the target protein is expressed intracellularly, the cells first need to be harvested by centrifugation before being resuspended in an appropriate lysis buffer

Low folate status and HHcy can activate microglia and astrocytes, leading to the release of pro-inflammatory cytokines (e.g., tumor necrosis factor-alpha (TNF-) and interleukin 6 (IL-6) [80]