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Preparation of cell lysate of PBMC Isolated PBMC (8 10 6 ) was suspended in 100 l hypotonic buffer (1.5 mM MgCl 2 , 10 mM KCl, 1 mM dithiothreitol, 10 mM HEPES, pH 7.9) containing protease inhibitor cocktail and sonicated

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The legal age for purchasing and using e-cigarettes is 18 years (Riigikogu, 2005)