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Microsyst Nanoeng 11 , 61 (2025)

2015;17:e23844

Disruption of this barrier permits microbial translocation into the submucosa, triggering inflammatory cascades that contribute to the pathogenesis of intestinal disorders such as IBD (159)

Semaglutide was initiated at 0.25 mg per week for the first 4 weeks via a pre-filled pen injector, escalating in a fixed-dose regimen every 4 weeks to reach the maintenance dose of 2.4 mg by week 16 (lower maintenance doses were permitted if participants were unable to tolerate 2.4 mg) (Extended Data Fig

SAM 10 volumes of 0.2% formic acid in methanol was added to the sample and incubated for 30 minutes followed by a spin at 14000 g for 10 minutes